Philippine Science High School Central Luzon Campus
ANTIBACTERIAL ACTIVITY OF Premna odorata Blanco (ALAGAW) LEAF EXTRACT ON Bacillus subtilis, Escherichia coli, AND Staphylococcus aureus
ANGELITA B. PANGILINAN MARCH 2013
Antibacterial activity of Premna odorata Blanco (Alagaw) Leaf Extract on Bacillus subtilis, Escherichia coli, and Staphylococcus aureus
by Angelita B. Pangilinan
ABSTRACT Alagaw (Premna odorataBlanco) is an important medicinal plant that possesses cardio tonic, antibiotic, anti-coagulant, carminative, hepatoprotective, and anti-tumor properties. It is proven to loosen phlegm and coughs. Literature showed that the genus Premna contains antimicrobial and ...view middle of the document...
___________________ ARIES N. OLIVEROS Chief, Curriculum and Instruction Services Division
This research work is hereby officially accepted as partial fulfillment of the requirements in Science and Technology Research II. _______________________ LILIA T. HABACON Director, Philippine Science High School – Central Luzon Campus
ACKNOWLEDGEMENTS I would like to thank the following, for without them, I would not have been able to complete this research project: To the PSHS – CLC, including its faculty and staff, who gave me the opportunity to conduct a research that would help the community; To my research adviser, Mr. Ralph S. Hipolito, who helped in order to make this research a better one; To the laboratory assistant, Ms. Angelica Mangubat, who supervised me in my laboratory works; To Jan Carlo L. Lima, Aaron Joseph Ilaya, Ivan Noriel Miranda, Roma Elaine Cruz and Katerine Rose Joson, who helped during the experimentation; To my parents for the inspiration, motivation and financial assistance; To the Department of Science and Technology – Region III and its employees, who processed the extract; And above all, to the ALMIGHTY FATHER who helped me in conducting and finishing my research.
Angelita B. Pangilinan
TABLE OF CONTENTS Page ABSTRACT APPROVAL SHEET ACKNOWLEDGEMENT TABLE OF CONTENTS LIST OF TABLES LIST OF FIGURES I. INTRODUCTION A. Background of the Study B. Statement of the Problem C. Significance of the Study D. Scope and Limitations II. REVIEW OF RELATED LITERATURE A. Premna odorata B. Bacillus subtilis C. Escherichia coli D. Staphylococcus aureus III. RESEARCH METHODOLOGY A. Process Flow Chart B. Preparation of P. odorata Blanco leaves 7 8
i ii iii iv vi vii
1 2 2 2
4 5 5 6
C. Preparation of Treatments D. Application and Data Gathering IV. V. VI. VII. VIII. RESULTS AND DISCUSSION SUMMARY AND CONCLUSION RECOMMENDATION LITERATURE CITED APPENDIX
8 9 12 15 16 17 19
LIST OF TABLES Table 1 2 3 4 5 6 Title Page
Zones of inhibition formed byPremna odorata B. extract on the Bacterial strains 9 Zones of Inhibition formed by Premna odorata B. extract on B. subtilis Zones of Inhibition formed by of Premna odorata B. extract on E. coli Zones of Inhibition formed by of Premna odorata B. extract on S. aureus Statistical Data of ANOVA Descriptive Statistics 17 17 18 18 19
LIST OF FIGURES Figure 1 2 3 4 5 P. odorata General Procedure Employed in the Study Graph of Efficiency of the Zones of inhibition Amoxicillin solution Bacterial subcultures Title Page 3 6 11 20 20
INTRODUCTION Background of the Study The genus Premna which is distributed in tropical and subtropical Asia, Africa, Australia, and the pacific Islands, is composed of 50 – 200 species (Yadav et al., 2010). Premna odorata Blanco, commonly known as alagaw is common in secondary forest at low altitude. This species is indigenous to the Islands of the Philippines (Yadav et al., 2010). P. odorata is one of the oldest...