Bio Ia Ensymes Essay

2000 words - 8 pages

The Effect of Enzyme Concentration on Rate of Reaction of Amylase, on the Hydrolysis of Starch into Maltose
Biology Internal Assessment - Labs

The Effect of Enzyme Concentration on Rate of Reaction of Amylase, on the Hydrolysis of Starch into Maltose
Biology Internal Assessment - Labs


Levie Lie
Levie Lie

Research Question
To investigate the effect of enzyme concentration (manipulated by increasing the concentration of enzyme amylase) on rate of reaction of amylase, on the hydrolysis of starch into maltose.

Enzymes are biological catalysts that catalyze biochemical reactions in living cells. In an enzyme-catalyzed reaction, the substrate ...view middle of the document...

The hypothesis for this experiment is that the rate of reaction will increase with the increase of enzyme amylase concentration, if the other factors of enzyme activity (such as temperature, pH, substrate concentration, inhibition) are kept constant. However, the rate of reaction will stop increasing with the increase of enzyme amylase concentration at a point where the substrate concentration becomes a limiting factor. At high enzyme concentration, all the substrate molecules available are saturated with the enzyme molecules at any given time; a further increase in enzyme concentration will not cause the increase in the rate of reaction.

As such, the expected graph from this experiment is as follows:

Variable | Independent/Dependent/controlled | Value | Strategy for altering, measuring or controlling |
Time taken for the solution to turn purple-black to colorless | Dependent | | The stopwatch is started once the amylase is added to the solution of starch and iodine. The stopwatch is stopped once the solution had turn completely colorless. |
Concentration of enzyme amylase | Independent | | Enzyme amylase solutions of different concentrations are prepared by adding different volumes of water to different volumes of 1% amylase solution. |
Substrate concentration | Controlled | 1% | The substrate concentration is kept constant at 1% starch solution volume is kept constant at 2cm3. |
Substrate volume | | 2cm3 | The substrate volume affects the time taken for enzyme to react on it thus it is kept at 2cm3 when adding it to the test tubes. |
Volume of enzyme amylase solution | | 2cm3 | Volume of enzyme amylase is kept constant at 2cm3. Controlling the volume of amylase ensures that the same amount of amylase molecules is available for reaction in the test tube. |
Size of test tubes | | 20 x 150 mm | The test tubes for each run must be of the same size and length. This is so the surface area exposed to the water bath is the same. |
Temperature | | 40°C | Enzyme activity is affected by temperate. The experiment is carried out in a 40°C water bath to ensure that all the solution are exposed to the same temperature. |
Volume of iodine solution | | 3 drops | The volume of iodine solution affects the color change. Thus, I have made it constant by adding three drops to each test tube. |

* 10cm3 of 1% amylase
* 10cm3 of 1% starch
* Distilled water
* 10cm3 of 1% amylase
* 10cm3 of 1% starch
* Distilled water
* Thermostatically controlled water bath
* 5 Test tubes
* Test tube rack
* 5 Droppers
* Measuring cylinder
* Thermometer
* Stopwatch

1. Prepare 2cm3 of 1% starch solution in 5 test tubes.
2. Add 3 drops of iodine solution into each test tube.
3. The solution should turn purple-black.
4. Serial dilution of the 1% amylase solution was performed following the diagram below.




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