This website uses cookies to ensure you have the best experience. Learn more

Biochemical Testing Essay

2690 words - 11 pages

NAME AND COURSE SECTION: Michele McNeill, Camile Manradge – Section 704

TITLE AND NUMBER: Biochemical Testing of Microbes – Lab #6

Exercise #1: Methyl Red-Voges Proskauer Test

Exercise #2: Motility Testing

Exercise #3: Carbohydrate Fermentation Testing

PURPOSE: We will prepare an E. coli culture, and further explore and become familiar with differential staining techniques using the Gram stain. We will use Crystal Violet dye, Iodine, acetone-alcohol, and Safranin to stain cells and differentiate between Gram-negative and Gram-positive bacteria (S. epidermidis, L. acidophilus, S. cerevisiae, and E. coli). Is to identify organisms.

PROCEDURE:

Pre-Experiment Preparation: ...view middle of the document...

coli and S. epidermidis cultures in the refrigerator for use in future experiments.

• We then mixed one tablespoon of bleach into the yeast and L. acidophilus cultures, let them sit for 30 minutes, and then discarded them.

A. Methyl Red Test Results
Both tubes were instantaneously red in color and had positive red bands on both of them.

B. Methyl Red Test Results
Next the instant red reaction to the reagent indicates a positive MR - VP test result. With this results prove that there is presence of acid from glucose. It was very interesting difference between the two test cultures was how far down the color penetrated. The E. coli was completely red, while the S. Epidermidis had a band of light liquid. 

Voges Proskauer Test
The two remaining broth test tubes were primed for the MR-VP test with four (4) drops of Barritt's B reagent. Tubes were mixed gently after each addition for oxygen exposure for color to occur. The tubes required a half hour (30 minutes) before reading of results. While waiting for the results we encounter a small problem by getting the tubes to stay in place, but we managed somehow to make it work. We then sterilized the tubes in boiling water for future use.

Result Interpretation
The liquid in both tubes was diluted orange gradually. They both had pink bands at the top, but color indicates an inconclusive result, meaning there was a pH between 4.4 and 6.

Exercise, 1a. – Catalase Test

• We first disinfect our work area again with 10% bleach.

• We then labeled 2 microscope slides E.coli and Epidermidis.

• We then used a plastic inoculation to loop to transfer a sample of each organism to the matching slide.

• Then we added a drop of hydrogen peroxide to smear. We did mix it with the plastic loop.

Catalase Results
The results showed no bubbles in S. Epidermis and only a few tiny, short lived bubbles in E. coli. According to the lab manual, this showed that there were two negative results.

OBSERVATIONS/DATA TABLES:

Experiment #6 Exercise 1 and 2 - Methyl Red; Voges-Proskauer, and Catalase Test:
|Culture |Methyl Red, Ex.1 |Voges–Proskauer, Ex.1 |Catalase Test, Ex. 2 |
|E. coli |Positive, red. |Positive, red band present. |Negative, no bubbles. |
|S. epidermis |Positive, red. |Positive, red band present. |Negative, no bubbles. |

RESULTS/ANALYSIS:

QUESTIONS:

Experiment #6 Methyl Red; Voges-Proskauer, and Catalase Test:
Questions 

A. What is meant by the term biochemical profile?
Biochemical profile is a variety of biochemical tests. These various tests are used to measure the constituents of plasma and serum in blood. 

B. What metabolic end product does the MR test for?
The metabolic end product MR tests for are the different bacteria’s related to their...

Other Essays Like Biochemical Testing

Preterm Premature Rupture Of Membranes Essay

1153 words - 5 pages test, therefore other testing would be necessary for confirmation (Friedman, 2013). A microscopic exam of vaginal fluid called a “ferning test” can confirm the presence of amniotic fluid, which appears crystalized after drying on a slide (Ward & Hisley, 2009). Additional tests used to confirm PPROM are an amniocentesis with Indigo carmine dye infusion, which would confirm the ROM if dye is visualized in vaginal secretions (Friedman, 2013). Also

How Gmo's Affect Your Life Essay

2787 words - 12 pages , writes in the introduction to his book, Genetic Roulette about the many side effects of GMOs that were displayed during animal testing: Lab animals tested with GM foods had stunted growth, impaired immune systems, bleeding stomachs, abnormal and potentially cancerous cell growth in the intestines, impaired blood cell development, misshapen cell structures in the liver, pancreas, and testicles, altered gene expression and cell metabolism

Psychology P1 and M1

3095 words - 13 pages are many health care links to the Biological approach these being; medication, scans and genetic testing. The first link is medication this is because medication can change the biochemical such as dopamine, if suffering with depression, this is by taking anti-depressants. Scans are linked as MRI, CAT and PET are images of the brain leading to doctors finding and hopefully solving any problems related to the brain. Genetic testing can detect

Gle Biology

2392 words - 10 pages | | |I |* Compare the mass of the reactants to the mass of the products in a chemical reaction or physical change (e.g., biochemical processes, carbon dioxide-oxygen cycle, nitrogen cycle, decomposition and synthesis

How Will the World End

1839 words - 8 pages eliminate the activity of beta-glucocerebrosidase. Without enough of this enzyme, glucocerebroside and related substances can build up to toxic levels within cells. Tissues and organs are damaged by the abnormal accumulation and storage of these substances, causing the characteristic features of Gaucher disease. Diagnosis A definitive diagnosis is made with genetic testing. As there are numerous different mutations, sequencing of the beta

Water Pollution

2903 words - 12 pages physical tests of water include temperature, solids concentration like total suspended solids (TSS) and turbidity. Chemical testing See also: water chemistry analysis and environmental chemistry Water samples may be examined using the principles of analytical chemistry. Many published test methods are available for both organic and inorganic compounds. Frequently used methods include pH, biochemical oxygen demand (BOD), chemical oxygen

Water Pollution

2781 words - 12 pages solids (TSS)) and turbidity. Chemical testing See also: water chemistry analysis and environmental chemistry Water samples may be examined using the principles of analytical chemistry. Many published test methods are available for both organic and inorganic compounds. Frequently used methods include pH, biochemical oxygen demand (BOD),[22]:102 chemical oxygen demand (COD),[22]:104 nutrients (nitrate and phosphorus compounds), metals

How Do Biomedical Model and Biopsychological Model Influence the Medical Practices and Public Health?

2717 words - 11 pages medicine.4 Trends of modern research in biology, biomedicine and drugs discovery7 Modern biological and biomedical research in is driven by the 4M-model: By first formulating hypotheses in the form of a mathematical-statistical-computational Models (network, biochemical,…), via experimental design such as molecular genetics, cell engineering or sampling patients, (Manipulate), data are Measured (e.g. via high throughput technoloqies

‘Criminals Are Born Not Made.’ Discuss

2250 words - 9 pages Shawcross. A study conducted by Witkin, involved testing 4000 men for the presence of the extra Y chromosome (Leo and Castronovo, 1985). While only twelve cases presented the extra gene, these twelve men may be more inclined towards crime; this was not found to be violent crime. This evidence favours support for a strong relationship between the extra gene and criminal behaviour. However, the link between the XYY chromosome and specifically violent

Army Health, Can I Dip in a Building

2030 words - 9 pages agree to inpatient or partial program placement), or counseling by the unit commander. The primary care manager may recommend one or a combination of these referrals. Leaders must ensure that enrolled soldiers are attending sessions, getting random biochemical testing and breathalyzers, and participating in the program. The focus of the Army substance abuse program is to return the soldier to full duty as soon as possible or identify for

Emotional Disterbance

2373 words - 10 pages , 2005). Biological factors are genetic, biochemical, and neurological influences that interact and result in emotional disabilities. Schizophrenia, autism, attention-deficit/hyperactivity disorder (ADHD), and Tourette syndrome—a tic disorder characterized by involuntary muscular movements, vocalizations, and/or inappropriate verbal outbursts—all appear to have biological bases that interact with other factors and may contribute to emotional

Related Papers

Early Toxicity Testing Market Global Industry Analysis, Size, Share, Growth, Trends And Forecast, 2013 2019

897 words - 4 pages toxicity testing is performed on living animals, in in vitro technique testing is performed in laboratories using biochemical assays and cellular assays without animals whereas in in silico technique testing is done by data analysis on computer with the help of data mining and data modeling tools. Out of all these techniques, there are significant advancements happened in in vivo technique; while other two techniques are still in development stage

Serratia Marcescens By Carter Stanley Is A Background Research Paper For A Science Fair Project Based On The Virus Serratis Marcescens And Its Antibiotics Tetracycline And Streptomycin

1171 words - 5 pages SERRATIA MARCESCENSSerratia marcescens is a pathogen that at one time, was considered to be harmless. However, testing conducted in the 20th century accidentally revealed just the opposite. The pivotal study called "Operation Sea Spray" was conducted in the early 1950's. The United States Army was testing how wind currents affected the spread of biochemical weapons. Giant balloons were filled with Serratia marcescens and floated over the city of

Why We Should Be More Cautious When Handling Money

1296 words - 6 pages school children. The twenty-shilling coin was selected for sampling because it is widely circulated. This coin is 25mm. Five coins were randomly taken from people in each category. Individuals would drop the coin into a 30 mL sterile container. Testing all the coins took place in different solutions and with different procedures. Bacterial colonies in each medium were characterized on the basis of colonial, cellular morphology, and staining

Radioimmunoassay Market Expected To Expand At A Steady Cagr Through 2026

825 words - 4 pages Quantification of a specific protein in a complex sample is a valuable contribution in the field of medical science. Immunoassays use the highly specific antibodies to target specific molecule of interest and analyze its concentration in a sample. Thus, immunoassay can be defined as a biochemical procedure which is used to measure the concentration of specific antigen with a specific antibody. The first immunoassay was developed in 1959 wherein