HOW ENZYMES FUNCTION
DR. MERCEDES MONDECAR
Enzymes are proteins produced by living cells, which regulate chemical reactions in the body.
Enzyme activity can be affected by
The experiments conducted were done to show that under optimum conditions; such as the ideal temperature, the right pH, and a higher enzyme or substrate concentration, the activity of the catalase enzyme would increase.
Exercise 5.1 Catalase Activity-Test tube one, which contains catalase buffered at pH 7 and hydrogen peroxide, will produce the highest bubble height because it contains the proper substrate/enzyme combo.
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Also, 10 mm of buffered catalase and 40 mm of hydrogen peroxide were used in test tube 1. For test tube 2, 20 mm of buffered catalase and 40 mm of hydrogen peroxide were used. And lastly, 30mm of buffered catalase and 40 mm of hydrogen peroxide were used in test tube 3.
Exercise 5.4 Effect of pH on Enzyme Activity- Material used for this part of the experiment also included three clean test tubes, and a ruler. For test tube 1, 10 mm of nonbuffered catalase, 20 mm of water with pH 3, and 40 mm of hydrogen peroxide were used. Materials needed for test tube 2 included, 10 mm of nonbuffered catalase, water with pH 7, and 40 mm of hydrogen peroxide. Lastly, 10 mm of nonbuffered catalase, 20 mm of water with pH11, and 40 mm of hydrogen peroxide were used in test tube 3.
COLLECTION OF DATA:
Exercise 5.1: Catalase Activity
This experiment was started by marking three clean test tubes at 1 cm and 5 cm levels with a black sharpie. In test tube 1, fill to the 1 cm mark with catalase buffered at pH 7 and hydrogen peroxide to the 5 cm mark. Shake test tube to mix ingredients, and wait at least 20 seconds for bubbling to start. Observe the reaction; then measure and record bubble height. Test tube 2, was filled with water to the 1 cm mark, and then hydrogen peroxide was filled to the 5 cm mark. The solution was mixed well for 20 seconds, no reaction was noted, and results were recorded. In the last test tube of this experiment, it was filled to the 1 cm mark with catalase and to the second mark with sucrose solution. The solution was mixed well, observed for 20 seconds, and a reaction was observed. The height of the bubble column was measured, and recorded.
Exercise 5.2 Effect of Temperature on Enzyme Activity
Start this experiment by marking three clean test tubes at 1 cm and 5 cm levels with a black sharpie. Next, fill each of the test tubes with catalase at pH 7 to the 1 cm mark. Then, place one of the tubes in a refrigerator, with the temperature at -10 degrees Celsius. Place another tube in an incubator, with the temperature set at 37...