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Investigation Of Action Of Saliva And Hcl

958 words - 4 pages

Practical 5

Investigation of Action of Saliva and Hydrochloric Acid in Two Carbohydrate
Solutions
__________________________________________________________________________
Objective:
Students are expected to state the objective of this experiment.
Apparatus & Equipments:
Boiling tubes
Graduated plastic dropper

Metal test tube racks
o
Water bath, ~ 37 C

Beaker
o
Water bath, ~ 95 C

Materials:
Carbohydrate solution A
3 M Hydrochloric acid

Carbohydrate solution B
3 M Sodium hydroxide

Benedict’s solution

Procedures:
1.
Prepare two boiling tubes containing 1 ml solution A and 1 ml solution B respectively. Add 1 ml
o
Benedict’s solution to each tube. Heat ...view middle of the document...

g., 1 into 1’, 2 into 2’ etc.). Straightaway, place back the
original tubes (labelled 1 to 4) back into the respective temperatures of incubation.

11.

Neutralize the acid in each of tube labelled 2’ and 3’ with 1 ml of sodium hydroxide (each). Shake
each tube (2’ and 3’) to ensure uniform mixing.

12.

Perform Benedict test on the contents of tubes 1’ to 4’ by pipetting 2 ml of Benedict solution into
o
each tubes and heating them in 95 C water bath for one minute. Record your observations in Table
2.

13.

After 35 minutes of incubating tubes 1 to 4, neutralize the acid in each test tube labelled 2 and 3
with 1ml of sodium hydroxide. Why is neutralization necessary?

o

30

Carry out Benedict’s test with an equal volume of Benedict’s solution (2 ml) for each tube.
Remember to heat your sample. Record your observations in Table 2.
Flow chart:
14.

1

Pour out for
Benedict’s test
Pour to

3

4

Solution B
2 ml
Incubate at
Mix with
Incubate at
After 5 mins

2

Solution B
2 ml

Solution B
2 ml

Solution B
2 ml

37°C
2 ml saliva
37°C

37°C
2 ml HCL
2 ml HCL
37°C
95°C
Remove tube 1, 2, 3, 4 from water bath.

2 ml saliva
95°C

2 ml

2 ml

2 ml

2 ml

1’

2’

3’

4’

Place tube 1,2,3,4 back into water bath for continuous incubation
NaOH
Benedict’s
solution

1 ml
2 ml

2 ml

1 ml
2 ml

2 ml

Heat for 1 min
Record observation at Table 2 (After 5th min)
After 35 mins
Remaining
content to
perform
Benedict’s test
NaOH
Benedict’s
solution

Remove tube 1, 2, 3, 4 from water bath.
2 ml

2 ml

2 ml

2 ml

2 ml

1 ml
2 ml

1 ml
2 ml

2 ml

Heat for 1 min
Record...

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