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Report On Drosophila

2308 words - 10 pages

Investigation of the inheritance of the characteristics eye colour and wing size in Drosophila melanogaster and whether the alleles are dominant or recessive and sex-linked or autosome

Date: 01-01-2014
Students: Janneke Hummelink & Reinder Meinsma

Table of Contents

* Theoretical background
* Introduction
* Materials
* Methods
* Results
* Conclusion
* Discussion
* Sources
* Logbook

Theoretical background
For at least for the past 100 years, the drosophila melanogaster has been one of the most famous organism in biology research. We all know this little organism with ...view middle of the document...

When you see an ‘egg’ popping out, you can be sure that this fly is female.

The aim of this practical assignment is to study two characteristics (white eyes and vestigial wings) of drosophila melanogaster, by crossbreeding flies with these characteristics. And to find out whether these characteristics are sex linked and whether the alleles are recessive or dominant. Hence our questions:
1. Is the allele in fruitflies for white eyes dominant or recessive and is this inheritance sex-linked?
2. Is the allele in fruitflies for vestigial wings dominant or recessive and is this inheritance sex-linked?
1. The allele for white eyes in fruitflies is recessive and its inheritance is sex-linked.
2. The allele for vestigial wings is recessive and its inheritance is not sex linked.
We created these hypotheses, because we basically had to come up with hypotheses and we did not know anything the coming two generations yet.
If our hypotheses are correct, the F1 generation will have a 100% chance of having red eyes. In our F2, the males will have a 50/50 chance of having either white eyes or red eyes. But the females do not have a chance of having white eyes.
The F1 generation will have a 100% chance of having normal wings and our F2 will have a 75/25 chance of having normal wings.

If my hypotheses are wrong, the assumptions are:
* White eye colour recessive and on X-chromosome
* Normal wing size recessive and on autosome

1. Eye colour: 2. Wing size:

P ♀ vg x ♂ wh -> P ♀ vg x ♂ wh ->

♀↓|♂→ | Y | Xe | | | | | ♀↓|♂→ | W | W |
XE | XEY | XEXe | | | | | w | Ww | Ww |
XE | XEY | XEXe | | | | | w | Ww | Ww |

F1 ♀ red x ♂ red -> F1 ♀ normal x ♂ normal->

♀↓|♂→ | XE | Y | | | | | ♀↓|♂→ | W | w |
XE | XEXE | XEY | | | | | W | WW | Ww |
Xe | XEXe | XeY | | | | | w | Ww | ww |

(reciprocal cross)

P ♀ wh x ♂ vg -> P ♀ wh x ♂ vg ->

♀↓|♂→ | XE | Y | | | | | ♀↓|♂→ | w | w |
Xe | XEXe | XeY | | | | | W | Ww | Ww |
Xe | XEXe | XeY | | | | | W | Ww | Ww |

F1 ♀ red x ♂ white -> F1 ♀ normal x ♂ normal ->

♀↓|♂→ | Xe | Y | | | | | ♀↓|♂→ | W | w |
XE | XEXe | XeY | | | | | W | WW | Ww |
Xe | XeXe | XeY | | | | | w | Ww | ww |

1. Eye colour: normal ♀ white normal ♂ white
1. P ♀ vg x ♂ wh -> F1 100% : 0 % | 100 % : 0 %

2. F1 ♀ red x ♂ red -> F2 100 % : 0 % | 50 % : 50 %

3. P ♀ wh x ♂ vg -> F1 100 %: 0 % | 0 % : 100 %

4. F1 ♀ red x ♂ wh -> F2 50 % : 50 % | 50 % : 50 %

2. Wing size: Normal ♀ Vestigial Normal ♂ Vestigial
1. P ♀ vg x ♂ wh -> F1 100 % : 0 % | 100 % : 0 %

2. F1 ♀ nor x ♂ nor -> F2 75 % : 25 % | 75 % : 25 %

3. P ♀ wh x ♂...

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