The mutation found on the 6th amino acid can lead to the polymerization of the sickle cell hemoglobin resulting in less oxygen concentration in the capillaries.
The purpose of this experiment was to cut DNA with restriction enzymes and find the size of the DNA fragments. It was also to compare the structure of DNAs from a normal patient with sickle cell anemia. In order to get this done the normal persons DNA was paired with a sick persons DNA with the same restriction enzyme in both. After which a double digest was done with the both enzymes used in both DNA types. The enzymes used were BamHI and EcoRI because I wanted to accommodate the ...view middle of the document...
A straight line was then drawn through the plotted points and the ruler used to determine the size of each fragment from the digested DNA migrated in the gel. In my lab I had a total of four fragments. Measuring from largest to smallest the size of the fragments in base pairs were 15849bp, 7943bp, 4467bp, 3981bp, 708bp, 562bp and178bp . My original hypothesis which was â€œThe mutation found on the 6th amino acid can lead to the polymerization of the sickle cell hemoglobin resulting in less oxygen concentration in the capillariesâ€ can be evaluated and examined by this experiment due to the knowledge if the HbS mutation is present on the 6th amino acid, the DNA will not be cut and a single large fragment of DNA will remain.Also if the both DNA is cut in similar places in this could mean that the normal person has the gene for the disease.
In conclusion the lab was successful and the objectives were met. A graph was drawn to determine the size of the fragments from the digested DNA migrated in the gel. The hypothesis was tested it was found that based on the results (location of the bands) the person without sickle cell anemia had indication of the disease in their recessive genes because the bands in both the normal and the diseased person matched up.