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Study Notes For The Main Scientists That Participated In The Early Discovery Of Dna And The Early Studies About Dna

554 words - 3 pages

-Hershey and Chase showed that DNA is the hereditary material of phages-Hershey and Chase labeled protein in a T2 phage with a radioactive tag -Then they labeled DNA in a separate batch of phage with a radioactive isotope -Both batches of tagged phages were allowed to infect separate batches of E.Coli bacteria -Afterwards, both sets of E. Coli. were placed in separate blenders to shake loose any parts of the phages that remained outside the bacterial cells -The mixtures were then place in separate centrifuges and spun around to force the bacterial cells to form a pellet at the bottom of the centrifuge -The viruses and parts of viruses remained in the supernatant -Hershey and Chase found that the ...view middle of the document...

coli for several generations on a medium containing a heavy isotope of nitrogen (15N) -Bacteria incorporated the heavy nitrogen into their nucleotides and then into their DNA -Then they transferred the bacteria to a medium containing a lighter more common isotope of nitrogen -Any new DNA that the bacteria synthesized would be lighter than the other DNA made in the 15N medium -Results of centrifuging the bacterial DNA eliminated other two models for DNA replication and proved Semiconservative model

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