This website uses cookies to ensure you have the best experience. Learn more

The Concentration Of Hydrogen Peroxide And Speed Of The Rate At Which It Is Broken Down By Catalase

3312 words - 14 pages

The Concentration of Hydrogen Peroxide and Speed of The Rate at Which It is Broken Down by Catalase


To find out whether the concentration of Hydrogen Peroxide affects the
rate of decomposition of hydrogen peroxide when broken down by


Hydrogen peroxide is a liquid bi-product of many chemical reactions in
living things. It is toxic, so has to be broken down by the enzyme
catalase which is also found in most living things. E.g.

[IMAGE]Hydrogen peroxide catalase water + oxygen

2H2O2 (aq) 2H2 O + O2


The enzyme catalase (peroxidase) was the first enzyme discovered and
is one of the ...view middle of the document...

Enzymes work most efficiently at an optimum temperature and an optimum


[IMAGE]Rate of reaction a. without enzyme

[IMAGE][IMAGE][IMAGE]Volume of b. with enzyme



Time in minutes

NB rate of reaction is the actual number of substrate molecules
evolved per minute.

Volume of oxygen produced/evolved per minute is not in itself the rate
of reaction, but is indicative of the actual rate of reaction.














10 vol H2 O2 20 vol H2 O2

Twice the concentration gives twice the number of hydrogen peroxide
molecules in the same volume; therefore twice the volume of oxygen
will be produced. This is because the frequency of collision will also
be doubled, as there are double the number of hydrogen peroxide
molecules for the catalase to collide with.


* Homogenise the potato in a pestle and mortar

* I will take 10ml of hydrogen peroxide and place it in a measuring

* To the measuring cylinder I will add 1.5g of homogenised potato,
which contains the enzyme catalase.

· I will then time it for 1 minute, and take a reading to see how much
oxygen foam has been produced; I will do this again after 2 minutes,
then after three minutes.

* I will do this 5 times in total, once with each concentration. The
concentrations are 2.5 vol, 5 vol, 10 vol, 20 vol, and 25 vol.

* I will then repeat the entire experiment.

I have chosen 2.5 as it is the lowest reasonable concentration that
will show a result. 5, 10 and 20 vol as they are twice the
concentration of the one before it and 25 as it is the highest
concentration that is safe to use. Above 25vol the hydrogen peroxide
becomes corrosive and the experiment would have to be performed under
close teacher supervision in a fume cupboard. This is not possible.

To make the experiment fair I will keep everything the same and only
vary the concentration of hydrogen peroxide. I have used doubling
concentrations in the hope of seeing a pattern in my results.


10 ml 2.5 vol hydrogen peroxide

10 ml 5 vol hydrogen peroxide

10 ml 10 vol hydrogen peroxide

10 ml 20 vol hydrogen peroxide

10 ml 25 vol hydrogen peroxide

5 measuring cylinders

5 weighing boats

1 pestle and mortar

Safety glasses


Stop watch

Electronic balance




Variables - In this investigation, the variables that affect the
activity of the enzyme, Catalase, were considered and controlled so
that they would...

Other Essays Like The Concentration Of Hydrogen Peroxide And Speed Of The Rate At Which It Is Broken Down By Catalase

Investigating Some Factors Which Affect The Rate Of Reaction

945 words - 4 pages Investigating Some Factors Which Affect the Rate of Reaction Between Hydrochloric Acid and Sodium ThiosulphateBackground InformationReactions can be completed at different rates. The rate of reaction is measured by observing how quickly reactants are used up or how quickly products are forming. This can be calculated in three ways:oPrecipitation - When the products of the reaction is a precipitate, which will cloud the solution. Observing a

An Investigation To Find Out How The Concentration Of Hydrochloric Acid Affects The Rate Of Reaction With Calcium Carbonate

2741 words - 11 pages measuring tube (which has water in it) in case of a spillage. Prediction My prediction is that, the higher the concentration of hydrochloric acid, the faster the rate of reaction. This is due to the fact that they'll be more particles in the acid colliding with other particles causing chemical reactions to take place at a faster rate than it would be if there was less acid. This is called a successful collision

The Power Of Speed, Energy, Confidence, And Strength

1844 words - 8 pages stated in Inside Karate: " Without relaxation there is no speed, since you will be too tense and tight. Relaxation does not mean the loose relaxation of cooked spaghetti, it should be a calm relaxation that unites your concentration, focus and intention." To practice the rules for breaking targets, the martial artist needs training. Two forms of training are external, and internal (Hallander, "Breaking" 2). The external training is

For Many Ppoems in Light of This Statement.Eople Home Is Defined by the People Who Live There. Compare and Contrast the Ways in Which Home Is Represented in at Least Two Poems

772 words - 4 pages For many ppoems in light of this statement.eople home is defined by the people who live there. Compare and contrast the ways in which home is represented in at least two poems. Many people associate home with the people living in it. So for them home could be anywhere in the world as long as their loved ones are with them. But when that person dies one loses their sense of security and also tends to feel abandoned

Kinetic Parameters of Catalase

1283 words - 6 pages Kinetic Parameters of Catalase Aya Essam El Garhy Ola El Rakhawy Students in Biochemistry Lab at German University in Cairo Abstract The activity of catalase enzyme towards hydrogen peroxide is tested by measuring the amount of H2O2 consumed by the enzyme catalase (by determining the decrease in H2O2 concentration) within a time interval of 5 minutes. Then ending the reaction by the addition of H2SO4 (by changing the PH of the

How Is Food Broken Down?

1476 words - 6 pages to further break down the hotdog. Your liver adds a liquid known as bile which helps to neutralize the acids from your stomach, so that digestion can continue to take place.  |   | | By now, you are done playing with your friends, and are back at home watching television. The chyme is slowly moving further along in your small intestine. As it moves along, the process of digestion is almost complete. Your small intestine is lined with tiny

Rate of Photosynthesis and How It Varies

851 words - 4 pages content and concentration determines how fast the rate of photosynthesis is. The more carbon dioxide, the faster the rate of photosynthesis. This is because carbon dioxide s used the process and so, the more there is, the more chance of the reaction taking place, and so, the more frequently it does take place. As a result, the carbon dioxide is a factor which can be manipulated in order to determine the rate of

To investigate the trajectory of a small ball as it rolls off a surface which is

566 words - 3 pages paper. The ink-side of the carbon paper should be facing down. 6. When a ball is released at the top of the ramp, the ball was traveled through a trajectory as shown in figure above. Theory: Let: g = 9.80 ms-2 u = speed of the ball as it leaves the ramp k = constant y = vertical distance (between the bottom of the ramp and the top of the board) x = horizontal distance (between the plum-line and mark on the paper] The

What Is Change And How Is It Represented And Linked In The Four Texts - Here Without You By 3 Doors Down, Spring Hail And The Widower In The Country By Les Murray And Sky High By Hannah Robert?

1517 words - 7 pages Change is inevitable, yet it can be denied depending on who and when they experience this change and also on the passing of time. Time can bring forth wanted and unwanted change. In 'Sky High' by Hannah Robert, Les Murray's 'Spring Hail' and 'The Widower in the Country' and 'Here Without You' by 3 Doors Down all demonstrate various aspects of how change is due to the passing of time, which is inevitable.In the text, 'Sky High' by Hannah Robert

Chapters 7-8 Study Questions: Lord Of The Flies By William Golding (If You Like The Essay, Please Rate It Good, And I Will E-Mail You The Original [Free Of Errors Caused By The Wesite] Copy)

1141 words - 5 pages words and actions, because he now turns to Piggy for guidance, not trusting his own judgment as he previously had. Additionally, Ralph seeks acceptance within the group of boys by demanding attention, a concept that was foreign to him before: ??I hit him,? said Ralph indignantly. ?I hit him with my spear, I wounded him.? He tried for their attention? (125). It is obvious that Ralph is now strating weaknesses in his role as leader, which may allow

Text Messaging Is Slows Down The Development Of Literacy

1867 words - 8 pages . Text messaging is dumbing down literacy for future generations. The newer generations have not had the privilege of experiencing a society that is not wrapped up in text based communication, such as instant messaging, chatting, and text messaging. Determining the effects of text messaging when it comes to college students and their ability to spell, has resulted in a positive nature. Shaw, Carlson, and Waxman (2007), performed a study on eighty

Related Papers

Investigation Of Enzymes And Hydrogen Peroxide

1282 words - 6 pages to denature. I found that the optimum for a catalase is at least 40°C. The enzyme denatured at about 60°C because the weak bonds, which hold the molecule into the specific shape for one substrate, are broken. The increase in molecular collisions and vibrations at higher temperatures is great enough to permanently change the shape of the active site. The enzyme is said to be denatured because it can no longer form an enzyme-substrate

Effect Of Concentration On The Rate Of Reaction

671 words - 3 pages + 2SO42- (1b) It is important however to note that the NH4 and K ions are still in the solution, they are just unreactive. In order to measure the rate of the reaction, the conventional method would be to measure the species in question at certain times. However, this would be inconvenient, especially for a three hour laboratory period. Since the iodide ion can be oxidized by the persulphate ion, we can use sodium thiosulphate to be an

The Speed Of Film Essay

1632 words - 7 pages Joshua Sissoyev Period 3 Ms. Schulz 10.19.2010 Career Biography Multi-Media Production “The Speed Of Film” Imagine a world of utter spectacular perfection, so beautiful and amazing that one must share it with everyone possible. The only problem is that they cannot see the creation that is locked in your mind. Sure one could describe these tales through vivid writing, but it will still seem to lack substance. It is said that, “One picture

The Effects Of Concentration Of Sugar On The Respiration Rate Of Yeast

1555 words - 7 pages respiration by yeast is generally called fermentation. Yeast is a living organism that produces enzymes. These enzymes break down glucose (by colliding with each other) to be able to respire anaerobically. I predict that the rate of fermentation will increase proportionally as the concentration of sugar increases but only up to a certain point were it will begin to decrease and eventually stop. I believe this